Visualizing spatiotemporal pattern of vascularization by SWIR fluorescence imaging in a mouse model of perforator flap transplantation

Visualizing spatiotemporal pattern of vascularization by SWIR fluorescence imaging in a mouse model of perforator flap transplantation

Blog Article

Abstract Vascularization as a spatiotemporally interlaced process involving angiogenesis and vascular remodeling, has seldom been investigated comprehensively regarding the interrelationship of the two intertwining but sequential processes.Here, a shortwave infrared (SWIR) fluorescence imaging strategy based on quantum dots (QDs) was designed to dynamically visualize vascularization in vivo and in situ in a perforator EnlargementGear transplantation mouse model.The vascularization process could be directly perceived from the established flap model with an optimal observation window at 10 min post-injection.

Anchored in SWIR technology and image processing, it was revealed that temporally, angiogenesis lasted throughout 21 days after surgery while vascular remodeling took a dominant role after 14 days both in vivo and in situ.Moreover, four perforasomes of the flap in situ displayed 4 in 1 Can Cooler spatially that Zone IV shortened the vascularization process with sufficient blood supply from the LDCIA, while Zone II recovered slowly from ischemia with a lack of blood supply.This study serves as a pioneer in adding novel cognition to spatiotemporal pattern of vascularization through visualizing angiogenesis and vascular remodeling simultaneously and dynamically, thus facilitating further investigation into the mechanisms behind.

Graphical Abstract.